Detection of a Novel Human Coronavirus by Real-Time Reverse-Transcription Polymerase Chain Reaction

Euro Surveill. 2012 Sep 27;17(39):20285. doi: 10.2807/ese.17.39.20285-en.

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coronavirus 229E, Human / genetics
  • Coronavirus 229E, Human / isolation & purification
  • Coronavirus Infections / diagnosis
  • Coronavirus Infections / genetics
  • Coronavirus Infections / virology*
  • Coronavirus NL63, Human / genetics
  • Coronavirus NL63, Human / isolation & purification
  • Coronavirus OC43, Human / genetics
  • Coronavirus OC43, Human / isolation & purification
  • Humans
  • Open Reading Frames
  • Real-Time Polymerase Chain Reaction / methods*
  • SARS Virus / genetics*
  • SARS Virus / isolation & purification*
  • Saudi Arabia
  • Sensitivity and Specificity
  • Travel
  • Viral Envelope Proteins

Substances

  • E protein, SARS coronavirus
  • Viral Envelope Proteins