Differential effect of allogeneic versus syngeneic mesenchymal stem cell transplantation in MRL/lpr and (NZB/NZW)F1 mice

Clin Immunol. 2012 Nov;145(2):142-52. doi: 10.1016/j.clim.2012.08.012. Epub 2012 Sep 17.


MSC are being explored as a promising novel treatment for SLE. In this study, we: 1) assessed the differential effects of allogeneic versus syngeneic MSC transplantation on lupus-like disease, 2) explored the mechanisms by which MSC modulate disease, and 3) investigated whether lupus-derived-MSC have intrinsic immunomodulatory defects. We showed that in MRL/lpr mice and (NZB/NZW)F1 mice, both B6-MSC and lupus-MSC from young mice ameliorated SLE-like disease and reduced splenic CD3+CD4+ T lymphocytes and CD19+CD21+ B lymphocytes. However, lupus-MSC from older (NZB/NZW)F1 mice did not reduce spleen weights, glomerular IgG deposits, renal pathology, interstitial inflammation, CD3+CD4+ T lymphocytes or CD19+CD21+ B lymphocytes significantly. Thus MSC transplantation ameliorates SLE-like disease partly through decreasing CD4+ T cell and naïve mature B cell numbers. Allogeneic MSC may be preferred over syngeneic lupus-derived-MSC given the decreased overall effectiveness of post-lupus-derived-MSC, which appears partially due to disease and not exclusively intrinsic defects in the MSC themselves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Antigens, CD / biosynthesis
  • Antigens, CD / immunology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / pathology
  • Female
  • Kidney Glomerulus / immunology*
  • Kidney Glomerulus / pathology
  • Lupus Erythematosus, Systemic / immunology
  • Lupus Erythematosus, Systemic / pathology
  • Lupus Erythematosus, Systemic / therapy*
  • Lymphocyte Count
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / immunology*
  • Mesenchymal Stem Cells / pathology
  • Mice
  • Mice, Inbred MRL lpr
  • Mice, Inbred NZB
  • Spleen / immunology
  • Spleen / pathology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / pathology
  • Transplantation, Homologous
  • Transplantation, Isogeneic


  • Antigens, CD