Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

Nat Methods. 2012 Nov;9(11):1077-80. doi: 10.1038/nmeth.2205. Epub 2012 Oct 7.


We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.

MeSH terms

  • Computational Biology / methods*
  • Escherichia coli / metabolism
  • Gene Regulatory Networks*
  • Genes, Synthetic*
  • Synthetic Biology*