Effect of polyphenols extracts from Brassica vegetables on erythrocyte membranes (in vitro study)

Environ Toxicol Pharmacol. 2012 Nov;34(3):783-90. doi: 10.1016/j.etap.2012.09.008. Epub 2012 Sep 23.


The aim of this work was to estimate the in vitro effects of polyphenol extracts from Brassica vegetables (Brussels sprouts and red cabbage) on erythrocyte membranes with normal and high concentration of cholesterol. To determine the effect of phenolic compounds we prospectively studied cholesterol concentration, lipid peroxidation, membrane fluidity and ATPase activity. Polyphenol extracts from Brassica vegetables resulted in statistically significant reductions in cholesterol concentrations in hypercholesterolemic erythrocytes. For control erythrocytes, no significant reduction of cholesterol levels was observed for both extracts. Decreases in lipid peroxidation intensity were observed after incubation of hypercholesterolemic erythrocytes with the extracts. No changes in membrane fluidity for both extracts were noted for normal and hypercholesterolemic erythrocytes. The activity of ATPase decreased after incubation of normal and hypercholesterolemic erythrocytes with extract from Brassica vegetables. Our results indicate that polyphenols from red cabbage and Brussels sprout may directly influence erythrocyte membrane properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Anticholesteremic Agents / pharmacology*
  • Brassica
  • Cholesterol / metabolism
  • Erythrocyte Membrane / drug effects*
  • Erythrocyte Membrane / metabolism
  • Humans
  • Lipid Peroxidation / drug effects
  • Membrane Fluidity / drug effects
  • Middle Aged
  • Plant Extracts / pharmacology*
  • Polyphenols / pharmacology*


  • Anticholesteremic Agents
  • Plant Extracts
  • Polyphenols
  • Cholesterol
  • Adenosine Triphosphatases