In vitro generation of platelets through direct conversion: first report in My Knowledge (iMK)

Cell Res. 2013 Feb;23(2):176-8. doi: 10.1038/cr.2012.142. Epub 2012 Oct 9.


Blood transfusion medicine requires a constant supply of platelets, which is now totally donor dependent. Recent advances in the generation of platelets in vitro through megakaryocytes (MKs) may provide protocols not only via pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), but also via induced MKs (iMKs). For the first time, mouse and human fibroblasts are successfully transdifferentiated into iMKs by the introduction of three factors, p45NF-E2, Maf G, and Maf K.

MeSH terms

  • 3T3 Cells
  • Animals
  • Blood Platelets / cytology
  • Cell Line
  • Cell Transdifferentiation*
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Megakaryocytes / cytology*
  • Megakaryocytes / metabolism
  • Mice
  • NF-E2 Transcription Factor, p45 Subunit / genetics
  • NF-E2 Transcription Factor, p45 Subunit / metabolism
  • Proto-Oncogene Proteins c-maf / genetics
  • Proto-Oncogene Proteins c-maf / metabolism
  • Proto-Oncogene Proteins c-myc / metabolism


  • NF-E2 Transcription Factor, p45 Subunit
  • Proto-Oncogene Proteins c-maf
  • Proto-Oncogene Proteins c-myc