Jagged-1-mediated activation of notch signalling induces adipogenesis of adipose-derived stem cells

Cell Prolif. 2012 Dec;45(6):538-44. doi: 10.1111/j.1365-2184.2012.00850.x. Epub 2012 Oct 10.

Abstract

Objectives: Notch signalling plays an important role in many cell activities, involving proliferation, migration, differentiation and cell death. The aim of this study was to investigate effects of such signalling on adipogenesis of mouse adipose-derived stem cells (mASCs).

Materials and methods: Jagged1 (50 and 100 ng/ml) was added to mASCs to activate Notch signalling, 2 days before adipogenic induction. At 5 and 7 days after induction, oil red-O staining was performed to evaluate lipid accumulation. Then real-time PCR was performed to examine expression of Notch downstream genes (Notch-1, -2, Hes-1 and Hey-1) and adipogenic transcription factor (PPAR-γ). Expressions of Hes-1 and PPAR-γ at protein level were confirmed by immunofluorescence staining.

Results: Our data indicated that Jagged1 promoted adipogenic differentiation of mASCs. Moreover, Jagged1 also increased expression of Notch downstream genes and PPAR-γ. Expressions of Hes-1 and PPAR-γ were found to be enhanced in Jagged1 pre-treated mASCs when compared to controls.

Discussion: The results led to the conclusion that activation of Notch signalling had stimulated adipogenesis of mASCs in the presence of adipogenic medium by promoting expression of PPAR-γ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / metabolism
  • Adipogenesis*
  • Adipose Tissue / cytology*
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Calcium-Binding Proteins / administration & dosage
  • Calcium-Binding Proteins / metabolism*
  • Cells, Cultured
  • Homeodomain Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / administration & dosage
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Jagged-1 Protein
  • Membrane Proteins / administration & dosage
  • Membrane Proteins / metabolism*
  • Mice
  • PPAR gamma / genetics
  • Rats
  • Receptors, Notch / genetics
  • Receptors, Notch / metabolism*
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / metabolism
  • Repressor Proteins / genetics
  • Serrate-Jagged Proteins
  • Signal Transduction
  • Stem Cells / cytology*
  • Stem Cells / metabolism
  • Transcriptional Activation

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Calcium-Binding Proteins
  • Hairy, HRT1 protein
  • Homeodomain Proteins
  • Intercellular Signaling Peptides and Proteins
  • Jag1 protein, mouse
  • Jag1 protein, rat
  • Jagged-1 Protein
  • Membrane Proteins
  • PPAR gamma
  • Receptors, Notch
  • Recombinant Proteins
  • Repressor Proteins
  • Serrate-Jagged Proteins