Differential effects of the protein kinase inhibitor K-252a on the in vitro survival of chick embryonic neurons

Neurosci Lett. 1990 Jan 1;108(1-2):207-12. doi: 10.1016/0304-3940(90)90732-o.


The effects of the protein kinase inhibitor K-252a on the in vitro survival of different populations of chick embryonic neurons were tested. Following dissociation from the corresponding ganglia by trituration, the neurons were cultured on laminin-coated dishes in both the presence and absence of their respective neurotrophic factor. K-252a alone promoted long-term neuronal survival of dorsal root ganglion (DRG) and ciliary ganglion (CG) neurons in a dose-dependent fashion. No such effect was seen with sympathetic ganglion (SG) neurons. In addition, K-252a did not interfere with nerve growth factor (NGF)- or ciliary neuronotrophic factor (CNTF)-mediated survival of DRG or CG neurons, respectively, but completely blocked the NGF- and CNTF-induced survival of SG neurons. High potassium-induced survival of SG neurons was not affected by K-252a. These results point to differences between various neuronal populations in the signal transduction mechanism for neurotrophic factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbazoles / pharmacology*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chick Embryo
  • Enzyme Inhibitors / pharmacology*
  • Ganglia, Parasympathetic / cytology
  • Ganglia, Parasympathetic / drug effects
  • Ganglia, Parasympathetic / enzymology*
  • Ganglia, Sympathetic / cytology
  • Ganglia, Sympathetic / drug effects
  • Ganglia, Sympathetic / enzymology*
  • Indole Alkaloids
  • Protein Kinases / metabolism*


  • Carbazoles
  • Enzyme Inhibitors
  • Indole Alkaloids
  • staurosporine aglycone
  • Protein Kinases