Complete Nucleotide Sequence of Plasmid pND6-2 From Pseudomonas Putida ND6 and Characterization of Conjugative Genes

Gene. 2013 Jan 1;512(1):148-56. doi: 10.1016/j.gene.2012.09.065. Epub 2012 Oct 6.

Abstract

Pseudomonas putida ND6 was shown to be capable of growth using naphthalene as sole carbon and energy sources. One plasmid from this strain, pND6-1, which was associated with the metabolism of naphthalene, was characterized; and the complete nucleotide sequence (101,858bp) and annotation of the plasmid were reported previously. In this paper, another plasmid (117,003bp) from P. putida ND6, pND6-2, was sequenced and 136 putative coding sequences (CDSs) were annotated. Among them, 9 CDSs were predicted to be involved in replication and partition of the plasmid, 32 CDSs encoded proteins associated with plasmid conjugative transfer, 2 CDSs encoded transcriptional regulators, 9 CDSs were necessary for DNA-associated function (including metabolism, recombination and repair), and 5 CDSs encoded proteins associated with other functions. The filter matting experiment indicated that pND6-2 is a helper plasmid, which could assist the naphthalene catabolic plasmid pND6-1 without any conjugative genes being transferred from P. putida ND6 to Escherichia coli AD256.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conjugation, Genetic*
  • DNA Repair / genetics
  • DNA Replication
  • Gene Order
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Open Reading Frames
  • Plasmids / genetics*
  • Pseudomonas putida / genetics*
  • Recombination, Genetic

Associated data

  • GENBANK/CP003589