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, 2 (1), 54

Dehalogenimonas Spp. Can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane

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Dehalogenimonas Spp. Can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane

Andrew D Maness et al. AMB Express.

Abstract

The contaminant concentrations over which type strains of the species Dehalogenimonas alkenigignens and Dehalogenimonas lykanthroporepellens were able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,1,2-trichloroethane (1,1,2-TCA) were evaluated. Although initially isolated from an environment with much lower halogenated solvent concentrations, D. alkenigignens IP3-3T was found to reductively dehalogenate chlorinated alkanes at concentrations comparable to D. lykanthroporepellens BL-DC-9T. Both species dechlorinated 1,2-DCA, 1,2-DCP, and 1,1,2-TCA present at initial concentrations at least as high as 8.7, 4.0, and 3.5 mM, respectively. The ability of Dehalogenimonas spp. to carry out anaerobic reductive dechlorination even in the presence of high concentrations of chlorinated aliphatic alkanes has important implications for remediation of contaminated soil and groundwater.

Figures

Figure 1
Figure 1
Experimentally measured ethene production as a function of initial aqueous-phase 1,2-DCA concentration after eight-weeks incubation ofD. alkenigignensIP3-3T(left) andD. lykanthroporepellensBL-DC-9T(right). Filled symbols indicate average of replicate bottles inoculated with bacterial strains. Open symbols indicate average of replicate uninoculated negative control bottles. Bars represent one standard deviation. Arrows denote concentration at and above which >1% of the starting 1,2-DCA remained at the end of the incubation period.
Figure 2
Figure 2
Experimentally measured propene production as a function of initial aqueous-phase 1,2-DCP concentration after eight-weeks incubation ofD. alkenigignensIP3-3T(left) andD. lykanthroporepellensBL-DC-9T(right). Filled symbols indicate average of replicate bottles inoculated with bacterial strains. Open symbols indicate average of replicate uninoculated negative control bottles. Bars represent one standard deviation. Arrows denote concentration at and above which >1% of the starting 1,2-DCP remained at the end of the incubation period.
Figure 3
Figure 3
Experimentally measured vinyl chloride production as a function of initial aqueous-phase 1,1,2-TCA concentration after eight-weeks incubation ofD. alkenigignensIP3-3T(left) andD. lykanthroporepellensBL-DC-9T(right). Filled symbols indicate average of replicate bottles inoculated with bacterial strains. Open symbols indicate average of replicate uninoculated negative control bottles. Bars represent one standard deviation. Arrows denote concentration at and above which >1% of the starting 1,1,2-TCA remained at the end of the incubation period.

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