Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic skunk cabbage (Symplocarpus renifolius)

Biosci Biotechnol Biochem. 2012;76(10):1990-2. doi: 10.1271/bbb.120434. Epub 2012 Oct 7.

Abstract

Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Araceae / enzymology*
  • Araceae / genetics*
  • Araceae / growth & development
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression Regulation, Plant*
  • Molecular Sequence Data
  • Papain / chemistry
  • Papain / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Papain