Possible role of the WDR3 gene on genome stability in thyroid cancer patients

PLoS One. 2012;7(9):e44288. doi: 10.1371/journal.pone.0044288. Epub 2012 Sep 26.

Abstract

The role of the WDR3 gene on genomic instability has been evaluated in a group of 115 differentiated thyroid cancer (DTC) patients. Genomic instability has been measured according to the response of peripheral blood lymphocytes to ionizing radiation (0.5 Gy). The response has been measured with the micronucleus (MN) test evaluating the frequency of binucleated cells with MN (BNMN), both before and after the irradiation. No differences between genotypes, for the BNMN frequencies previous the irradiation, were observed. Nevertheless significant decreases in DNA damage after irradiation were observed in individuals carrying the variant alleles for each of the three genotyped SNPs: rs3754127 [-8.85 (-15.01 to -2.70), P<0.01]; rs3765501 [-8.98 (-15.61 to -2.36), P<0.01]; rs4658973 [-8.70 (-14.94 to -2.46), P<0.01]. These values correspond to those obtained assuming a dominant model. This study shows for the first time that WDR3 can modulate genome stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma, Follicular / genetics*
  • Adenocarcinoma, Follicular / pathology
  • Adult
  • Alleles
  • Carcinoma / genetics*
  • Carcinoma / pathology
  • Carcinoma, Papillary
  • DNA Damage
  • Female
  • Gamma Rays
  • Gene Frequency
  • Genomic Instability*
  • Genotype
  • Humans
  • Leukocytes, Mononuclear / metabolism
  • Leukocytes, Mononuclear / pathology
  • Leukocytes, Mononuclear / radiation effects
  • Male
  • Micronucleus Tests
  • Middle Aged
  • Nuclear Proteins / genetics*
  • Polymorphism, Single Nucleotide*
  • Radiation Tolerance
  • Thyroid Cancer, Papillary
  • Thyroid Neoplasms / genetics*
  • Thyroid Neoplasms / pathology

Substances

  • Nuclear Proteins
  • WDR3 protein, human

Grant support

Spanish Ministry of Education and Science (SAF2007-6338) and the Generalitat de Catalunya (CIRIT; 2009SGR-725). W.A. García-Quispes was supported by a predoctoral fellowship from the Universitat Autònoma de Barcelona (PIF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.