Resting-state network disruption and APOE genotype in Alzheimer's disease: a lagged functional connectivity study

PLoS One. 2012;7(9):e46289. doi: 10.1371/journal.pone.0046289. Epub 2012 Sep 25.


Background: The apolipoprotein E epsilon 4 (APOE-4) is associated with a genetic vulnerability to Alzheimer's disease (AD) and with AD-related abnormalities in cortical rhythms. However, it is unclear whether APOE-4 is linked to a specific pattern of intrinsic functional disintegration of the brain after the development of the disease or during its different stages. This study aimed at identifying spatial patterns and effects of APOE genotype on resting-state oscillations and functional connectivity in patients with AD, using a physiological connectivity index called "lagged phase synchronization".

Methodology/principal findings: Resting EEG was recorded during awake, eyes-closed state in 125 patients with AD and 60 elderly controls. Source current density and functional connectivity were determined using eLORETA. Patients with AD exhibited reduced parieto-occipital alpha oscillations compared with controls, and those carrying the APOE-4 allele had reduced alpha activity in the left inferior parietal and temporo-occipital cortex relative to noncarriers. There was a decreased alpha2 connectivity pattern in AD, involving the left temporal and bilateral parietal cortex. Several brain regions exhibited increased lagged phase synchronization in low frequencies, specifically in the theta band, across and within hemispheres, where temporal lobe connections were particularly compromised. Areas with abnormal theta connectivity correlated with cognitive scores. In patients with early AD, we found an APOE-4-related decrease in interhemispheric alpha connectivity in frontal and parieto-temporal regions.

Conclusions/significance: In addition to regional cortical dysfunction, as indicated by abnormal alpha oscillations, there are patterns of functional network disruption affecting theta and alpha bands in AD that associate with the level of cognitive disturbance or with the APOE genotype. These functional patterns of nonlinear connectivity may potentially represent neurophysiological or phenotypic markers of AD, and aid in early detection of the disorder.

MeSH terms

  • Aged
  • Alzheimer Disease / genetics*
  • Alzheimer Disease / physiopathology*
  • Apolipoprotein E4 / genetics*
  • Apolipoproteins E / genetics*
  • Brain / metabolism
  • Brain / physiology*
  • Electroencephalography
  • Female
  • Genotype
  • Humans
  • Male
  • Parietal Lobe / metabolism
  • Parietal Lobe / physiopathology


  • Apolipoprotein E4
  • Apolipoproteins E

Grant support

The authors have no support or funding to report.