Preventive effects of Egyptian sweet marjoram (Origanum majorana L.) leaves on haematological changes and cardiotoxicity in isoproterenol-treated albino rats

Cardiovasc Toxicol. 2013 Jun;13(2):100-9. doi: 10.1007/s12012-012-9189-4.


We made an attempt to evaluate/compare the cardioprotective activity of two different doses (50 and 100 mg/kg body weight, given orally for 30 consecutive days) of Egyptian sweet marjoram leaf powder (MLP) and marjoram leaf aqueous extract (MLE) against isoproterenol (ISO)-induced myocardial infarcted rats (150 mg/kg body weight, twice at an interval of 24 h on days 29 and 30). The present study showed (probably for the first time) that both MLP and MLE (especially the high dose) significantly alleviated (P < 0.05-0.001) erythrocytosis, granulocytosis, thrombocytosis, shortened clotting time, the increase in relative heart weight, myocardial oxidative stress and the leakage of heart enzymes (creatine phosphokinase (CPK), CPK-MB isoenzyme, lactate dehydrogenase and aminotransferase) in ISO-treated rats through reactivating non-enzymic (reduced glutathione) and enzymic (catalase, glutathione peroxidase, glutathione S-transferase, superoxide dismutase) antioxidant defence system and inhibiting the production of nitric oxide and lipid peroxidation in heart tissues. The modulatory effects of marjoram leaves shown in the present study were dose-dependent in most cases and much higher in MLE (4.3-20.3 % for all parameters taken together). In addition, the doses used in the present study were considered safe. In conclusion, this study may have a significant impact on myocardial infarcted patients.

MeSH terms

  • Animals
  • Blood Coagulation / drug effects
  • Cardiotonic Agents / pharmacology*
  • Cardiotonic Agents / toxicity
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Enzymes / metabolism
  • Heart / drug effects
  • Hematologic Diseases / chemically induced
  • Hematologic Diseases / drug therapy*
  • Hematologic Diseases / metabolism
  • Isoproterenol / toxicity
  • Male
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / metabolism
  • Myocardium / enzymology
  • Myocardium / pathology
  • Origanum / chemistry*
  • Oxidative Stress / drug effects
  • Pancytopenia / chemically induced
  • Pancytopenia / drug therapy
  • Pancytopenia / metabolism
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Polycythemia / chemically induced
  • Polycythemia / drug therapy
  • Polycythemia / metabolism
  • Rats
  • Rats, Wistar
  • Thrombocytosis / chemically induced
  • Thrombocytosis / drug therapy
  • Thrombocytosis / metabolism
  • Whole Blood Coagulation Time


  • Cardiotonic Agents
  • Enzymes
  • Plant Extracts
  • Isoproterenol