Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF

PLoS One. 2012;7(10):e46857. doi: 10.1371/journal.pone.0046857. Epub 2012 Oct 8.


The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism*
  • Biofilms
  • Hemagglutination
  • Immobilized Proteins / chemistry
  • Immobilized Proteins / metabolism
  • Lectins / chemistry
  • Lectins / metabolism*
  • Protein Binding
  • Pseudomonas aeruginosa / cytology*
  • Pseudomonas aeruginosa / metabolism*
  • Pseudomonas aeruginosa / physiology
  • Substrate Specificity


  • Bacterial Proteins
  • Immobilized Proteins
  • LecB protein, Pseudomonas aeruginosa
  • Lectins
  • OprF protein, Pseudomonas aeruginosa