Abstract
We show that DNA containing a conformationally-locked anti analogue of O(6)-alkylguanine is a poor substrate for human O(6)-methylguanine-DNA methyltransferase (MGMT) and the alkyltransferase-like protein, Atl1. This highlights the requirement for the syn conformation and rationalises why certain O(6)-alkylguanines are poor MGMT substrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkyl and Aryl Transferases / metabolism*
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Crystallography, X-Ray
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Guanine / analogs & derivatives
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Guanine / metabolism*
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Humans
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O(6)-Methylguanine-DNA Methyltransferase / metabolism*
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Oligodeoxyribonucleotides / biosynthesis*
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Protein Binding
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Protein Structure, Tertiary
Substances
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Oligodeoxyribonucleotides
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Guanine
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O(6)-Methylguanine-DNA Methyltransferase
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Alkyl and Aryl Transferases