Synthesis, characterization and histomorphometric analysis of cellular response to a new elastic DegraPol® polymer for rabbit Achilles tendon rupture repair

J Tissue Eng Regen Med. 2015 May;9(5):584-94. doi: 10.1002/term.1624. Epub 2012 Oct 11.

Abstract

Tendon rupture repair is a surgical field where improvements are still required due to problems such as repeat ruptures, adhesion formation and joint stiffness. In the current study, a reversibly expandable and contractible electrospun tube based on a biocompatible and biodegradable polymer was implanted around a transected and conventionally sutured rabbit Achilles tendon. The material used was DegraPol® (DP), a polyester urethane. To make DP softer, more elastic and surgeon-friendly, the synthesis protocol was slightly modified. Material properties of conventional and new DP film electrospun meshes are presented. At 12 weeks post-surgery, tenocyte and tenoblast density, nuclei and width, collagen fibre structure and inflammation levels were analyzed histomorphometrically. Additionally, a comprehensive histological scoring system by Stoll et al. (2011) was used to compare healing outcomes. Results showed that there were no adverse reactions of the tendon tissue following the implant. No differences were found whether the DP tube was applied or not for both traditional and new DP materials. As a result, the new DP material was shown to be an excellent carrier for delivery of growth factors, stem cells and other agents responsible for tendon healing.

Keywords: DegraPol synthesis; carrier; drug delivery system; tendon; tendon repair; tendon rupture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achilles Tendon / surgery*
  • Animals
  • Drug Delivery Systems
  • Elasticity
  • Electrochemistry
  • Female
  • Materials Testing
  • Polyesters / chemical synthesis
  • Polyesters / chemistry*
  • Polyurethanes / chemical synthesis
  • Polyurethanes / chemistry*
  • Rabbits
  • Reconstructive Surgical Procedures
  • Regeneration
  • Regenerative Medicine / instrumentation
  • Rupture / pathology
  • Tendon Injuries / pathology*
  • Tendons / pathology
  • Tissue Scaffolds
  • Treatment Outcome
  • Wound Healing

Substances

  • DegraPol
  • Polyesters
  • Polyurethanes