Stimulated angiogenesis for fracture healing augmented by low-magnitude, high-frequency vibration in a rat model-evaluation of pulsed-wave doppler, 3-D power Doppler ultrasonography and micro-CT microangiography

Ultrasound Med Biol. 2012 Dec;38(12):2120-9. doi: 10.1016/j.ultrasmedbio.2012.07.025. Epub 2012 Oct 11.

Abstract

This study aimed to investigate the mechanism of low-magnitude high-frequency vibration (LMHFV) treatment on angiogenesis and blood flow for enhancement of fracture healing. Nine-month-old ovariectomized (OVX) and sham-operated (Sham) rats received closed fractures creation at the femora and were randomized into LMHFV treatment (Sham-V, OVX-V) or control (Sham-C, OVX-C) groups. Pulsed-wave Doppler indicated an increase in blood flow velocity of the femoral artery at weeks 2 (OVX pair: p = 0.030) and 4 (OVX pair: p = 0.012; Sham pair: p = 0.020) post-treatment. Significantly enhanced vascular volume (VV) at the fracture site in the vibration groups was demonstrated by 3-D high-frequency power Doppler at week 2 (Sham pair: p = 0.021) and micro-computed tomography (microCT) microangiography at weeks 2 (OVX pair: p = 0.009) and 4 (OVX pair: p = 0.034), which echoed the osteogenesis findings by radiographic and microCT analysis. VV in the OVX groups was inferior to the Sham groups. However, OVX-V showed higher percentages of angiogenic enhancement than Sham-V. Despite impaired neo-angiogenesis in osteoporotic fractures, LMHFV could increase blood flow and angiogenesis in both normal and osteoporotic fractures, thus enhancing fracture healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiography*
  • Animals
  • Bone and Bones / blood supply*
  • Female
  • Femoral Fractures / diagnostic imaging
  • Femoral Fractures / physiopathology
  • Femoral Fractures / therapy
  • Fracture Healing*
  • Immunohistochemistry
  • Neovascularization, Physiologic*
  • Osteoporotic Fractures / diagnostic imaging
  • Osteoporotic Fractures / physiopathology
  • Osteoporotic Fractures / therapy*
  • Rats
  • Rats, Sprague-Dawley
  • Ultrasonography, Doppler*
  • Ultrasonography, Doppler, Pulsed*
  • Vascular Endothelial Growth Factor A / metabolism
  • Vibration / therapeutic use*
  • X-Ray Microtomography*

Substances

  • Vascular Endothelial Growth Factor A