Immunodetection with a monoclonal antibody of glutathione S-transferase mu in patients with and without carcinomas

Biochem Pharmacol. 1990 Feb 1;39(3):591-7. doi: 10.1016/0006-2952(90)90068-v.

Abstract

Several monoclonal antibodies against human liver glutathione S-transferase mu were developed. One of these monoclonal antibodies, called GST-3H4 was further characterized and used in this study. In hepatic tissue, after immunoblotting, GST-3H4 strains a 27 kDa protein with a pI value of 6.2. GST-3H4 recognizes other human class-mu glutathione S-transferases, but does not detect acidic or basic glutathione S-transferases. By immunodetection with this monoclonal antibody, glutathione S-transferase mu can be demonstrated in human breast, stomach, liver, small and large intestine, mononuclear blood cells, kidney and placenta. A 100% correlation is found in the distribution of glutathione S-transferase mu when different tissues or mononuclear blood cells from the same individuals are investigated. In 62.5% of the mononuclear blood cells from controls, glutathione S-transferase mu is present. In patients with polyposis coli, breast cancer or colon cancer a similar distribution is found. Therefore no important role for glutathione S-transferase mu deficiencies in the aetiology of these diseases is suggested.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Blotting, Western
  • Breast Neoplasms / enzymology
  • Colonic Neoplasms / enzymology
  • Female
  • Glutathione Transferase / analysis*
  • Humans
  • Intestinal Mucosa / enzymology
  • Intestines / enzymology
  • Isoenzymes / analysis*
  • Leukocytes, Mononuclear / enzymology
  • Liver / enzymology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Neoplasms / enzymology*
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Isoenzymes
  • Glutathione Transferase