Mss116p: a DEAD-box protein facilitates RNA folding

RNA Biol. 2013 Jan;10(1):71-82. doi: 10.4161/rna.22492. Epub 2012 Oct 12.


RNA folding is an essential aspect underlying RNA-mediated cellular processes. Many RNAs, including large, multi-domain ribozymes, are capable of folding to the native, functional state without assistance of a protein cofactor in vitro. In the cell, trans-acting factors, such as proteins, are however known to modulate the structure and thus the fate of an RNA. DEAD-box proteins, including Mss116p, were recently found to assist folding of group I and group II introns in vitro and in vivo. The underlying mechanism(s) have been studied extensively to explore the contribution of ATP hydrolysis and duplex unwinding in helicase-stimulated intron splicing. Here we summarize the ongoing efforts to understand the novel role of DEAD-box proteins in RNA folding.

Keywords: DEAD-box proteins; RNA folding; RNA helicase; RNA-protein interactions; intron; ribozyme.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • DEAD-box RNA Helicases / chemistry
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Exons
  • Introns
  • Mitochondria / genetics
  • Mitochondria / metabolism
  • Mutation
  • Protein Binding
  • Protein Interaction Domains and Motifs / physiology
  • RNA / chemistry*
  • RNA / metabolism*
  • RNA Folding*
  • RNA Splicing
  • RNA Stability
  • Yeasts


  • RNA
  • DEAD-box RNA Helicases