Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jan;32(1):103-16.
doi: 10.1007/s00299-012-1345-6. Epub 2012 Oct 12.

Identification of QTLs Associated With Tissue Culture Response Through Sequencing-Based Genotyping of RILs Derived From 93-11 × Nipponbare in Rice (Oryza Sativa)

Affiliations

Identification of QTLs Associated With Tissue Culture Response Through Sequencing-Based Genotyping of RILs Derived From 93-11 × Nipponbare in Rice (Oryza Sativa)

Sujuan Li et al. Plant Cell Rep. .

Abstract

KEY MESSAGE : The performance of callus induction and callus differentiation was evaluated by 9 indices for 140 RILs; 2 major QTLs associated with plant regeneration were identified. In order to investigate the genetic mechanisms of tissue culture response, 140 recombinant inbred lines (RILs) derived from 93-11 (Oryza sativa ssp. indica) × Nipponbare (Oryza sativa ssp. japonica) and a high quality genetic map based on the SNPs generated from deep sequencing of the RIL genomes, were used to identify the quantitative trait loci (QTLs) associated with in vitro tissue culture response (TCR) from mature seed in rice. The performance of callus induction was evaluated by indices of induced-callus color (ICC), induced-callus size (ICS), induced-callus friability (ICF) and callus induction rate (CIR), respectively, and the performance of callus differentiation was evaluated by indices of callus proliferation ability (CPA), callus browning tendency (CBT), callus greening ability (CGA), the average number of regenerated shoots per callus (NRS) and regeneration rate (%, RR), respectively. A total of 25 QTLs, 2 each for ICC, ICS, ICF, CIR and CBA, 3 for CPA, 4 each for CGA, NRS and RR, respectively, were detected and located on 8 rice chromosomes. Significant correlations were observed among the traits of CGA, NRS and RR, and QTLs identified for these three indices were co-located on chromosomes 3 and 7, and the additive effects came from both Nipponbare and 93-11, respectively. The results obtained from this study provide guidance for further fine mapping and gene cloning of the major QTL of TCR and the knowledge of the genes underlying the traits investigated would be very helpful for revealing the molecular bases of tissue culture response.

Similar articles

See all similar articles

Cited by 7 articles

See all "Cited by" articles

References

    1. Mol Cells. 2007 Jun 30;23(3):323-30 - PubMed
    1. Plant J. 2006 Sep;47(6):969-76 - PubMed
    1. Mol Cells. 2001 Feb 28;11(1):64-7 - PubMed
    1. BMC Genomics. 2010 Nov 24;11:656 - PubMed
    1. Plant Cell Rep. 2009 Feb;28(2):247-56 - PubMed

Publication types

LinkOut - more resources

Feedback