The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica

FEMS Yeast Res. 2013 Feb;13(1):50-61. doi: 10.1111/j.1567-1364.2012.12008.x. Epub 2012 Oct 15.

Abstract

Tec1p in the budding yeast Saccharomyces cerevisiae is important for dimorphic transition. In this study, we identified a homologue of Tec1p, YlTec1p, in the distantly related dimorphic yeast Yarrowia lipolytica. YlTec1p contains an evolutionarily conserved TEA/ATTS DNA-binding domain. Expression of YlTEC1 in S. cerevisiae tec1Δ cells rescued the invasive growth defect and activated a FLO11-lacZ reporter, indicating that YlTec1p is functionally related to Tec1p. However, YlTEC1 expression failed to activate an FRE-lacZ reporter, suggesting that these two transcription factors are different. YlTEC1 plays a negative role in the yeast-to-hypha transition in Y. lipolytica based on gene deletion and overexpression studies. We show that YlTec1p activates rather than represses gene expression in Y. lipolytica by yeast one-hybrid assay, and YlTec1p is critical for the activation of FLO11-lacZ in Y. lipolytica. In addition, YlTec1p localized to the nucleus and its nuclear localization decreased during hyphal growth. We speculate that YlTec1p may normally regulate the expression of a set of target genes that may prevent rather than promote hyphal development in Y. lipolytica. Our study also suggests that YlTEC1 may not be largely regulated by the cAMP-protein kinase A pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Down-Regulation
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal*
  • Genes, Reporter
  • Genetic Complementation Test
  • Hyphae / cytology
  • Hyphae / genetics*
  • Hyphae / growth & development
  • Hyphae / physiology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Sequence Alignment
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Yarrowia / cytology
  • Yarrowia / genetics*
  • Yarrowia / growth & development
  • Yarrowia / physiology

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • TEC1 protein, S cerevisiae
  • Transcription Factors