Eradication of Helicobacter pylori infection by the probiotic strains Lactobacillus johnsonii MH-68 and L. salivarius ssp. salicinius AP-32

Helicobacter. 2012 Dec;17(6):466-77. doi: 10.1111/j.1523-5378.2012.00992.x. Epub 2012 Aug 28.


Background: The current therapy for Helicobacter pylori infection includes antimicrobial agents and proton pump inhibitors. We have examined the ability of Lactobacillus spp. to inhibit H. pylori infection.

Materials and methods: Probiotic strains isolated from samples of adult feces, infant feces, breast milk, and vaginal swab collected from healthy volunteers in Taiwan and commercially available strains were screened for antagonism toward H. pylori. Inhibition liquid culture assay was used to screen potential anti-H. pylori activity. Then, we performed agar plate inhibition assay, and assays to determine the capacity of probiotics for adhesion, and inhibition and killing of H. pylori, and measured the levels of IL-8 and IL-10. Using animal models, we studied regulation of gastric acid and histopathological changes accompanying anti-H. pylori activity.

Results: We found that six of the tested strains suppressed urease activity of H. pylori: Lactobacillus acidophilus TYCA08, L. acidophilus TYCA15, L. johnsonii MH-68, and L. salivarius subsp. salicinius AP-32 were more effective than the others. In vivo, L. johnsonii MH-68 and L. salivarius subsp. salicinius AP-32 alone or in combination, reduced the H. pylori load in the gastric mucosa, and also reduced inflammatory chemokine expression and lymphocyte infiltration.

Conclusions: Lactobacillus johnsonii MH-68 and L. salivarius subsp. salicinius AP-32 effectively suppress H. pylori viability, and when used as probiotics, they may help decrease the occurrence of gastritis, and even reduce the risk of H. pylori infection.

MeSH terms

  • Adult
  • Animals
  • Antibiosis
  • Bacterial Adhesion
  • Bacterial Load
  • Cell Line
  • Disease Models, Animal
  • Female
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / pathology
  • Helicobacter Infections / therapy*
  • Helicobacter pylori / growth & development
  • Helicobacter pylori / isolation & purification
  • Humans
  • Infant
  • Interleukin-10 / metabolism
  • Interleukin-8 / metabolism
  • Lactobacillus / isolation & purification
  • Lactobacillus / physiology*
  • Male
  • Mice
  • Milk, Human / microbiology
  • Probiotics / administration & dosage*
  • Rats
  • Rats, Sprague-Dawley
  • Taiwan
  • Treatment Outcome
  • Vagina / microbiology


  • Interleukin-8
  • Interleukin-10