The Junctional Adhesion Molecule-B regulates JAM-C-dependent melanoma cell metastasis

FEBS Lett. 2012 Nov 16;586(22):4046-51. doi: 10.1016/j.febslet.2012.10.005. Epub 2012 Oct 12.


Metastasis is a major clinical issue and results in poor prognosis for most cancers. The Junctional Adhesion Molecule-C (JAM-C) expressed by B16 melanoma and endothelial cells has been involved in metastasis of tumor cells through homophilic JAM-C/JAM-C trans-interactions. Here, we show that JAM-B expressed by endothelial cells contributes to murine B16 melanoma cells metastasis through its interaction with JAM-C on tumor cells. We further show that this adhesion molecular pair mediates melanoma cell adhesion to primary Lung Microvascular Endothelial Cells and that it is functional in vivo as demonstrated by the reduced metastasis of B16 cells in Jam-b deficient mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Cells, Cultured
  • Coculture Techniques
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism*
  • Female
  • Flow Cytometry
  • Immunoglobulins / genetics
  • Immunoglobulins / metabolism*
  • Immunohistochemistry
  • Lung / blood supply
  • Lung / metabolism
  • Male
  • Melanoma, Experimental / genetics
  • Melanoma, Experimental / metabolism*
  • Melanoma, Experimental / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Confocal
  • Neoplasm Metastasis
  • Protein Binding
  • RNA Interference


  • Cell Adhesion Molecules
  • Immunoglobulins
  • Jam2 protein, mouse
  • Jam3 protein, mouse