Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 33 (2), 496-505

Elevated Complement Factor H Levels in Asthmatic Sputa

Affiliations

Elevated Complement Factor H Levels in Asthmatic Sputa

Zsóka Weiszhár et al. J Clin Immunol.

Abstract

Background: The alternative pathway of the complement system is known to play a role in the generation of asthmatic airway inflammation, but its regulatory complement protein, factor H has not been investigated in this disease.

Purpose: Our aim was to determine the local bronchial complement factor H (CFH) levels in asthma, and to investigate its relationship with complement activation, systemic CFH concentrations and clinical characteristics of patients.

Methods: Induced sputum and plasma were collected from 21 healthy and 26 asthmatic subjects, and complement factor H and SC5b-9 concentrations were assessed by ELISA. Total protein concentrations were determined by biuret-reaction based microassay system from induced sputa.

Results: CFH was detectable in 81 % of healthy and 100 % of asthmatic subjects, while SC5b-9 exceeded the detection limit in 62 % of healthy subjects and 85 % of asthmatic patients. Sputum CFH concentrations and CFH/protein ratios were increased in samples from asthmatic patients, and correlated with loss of lung function, asthma control, severity and medication intensity, but not with plasma CFH concentrations. Sputum CFH/protein ratios were in positive correlation also with sputum eosinophilic cell counts in asthma. SC5b-9 concentrations were not higher in the asthmatic sputa, although they correlated with sputum CFH concentrations.

Conclusions: CFH level is elevated on asthmatic airway surface, and may be associated with uncontrolled inflammation in asthma.

Similar articles

See all similar articles

References

    1. J Clin Invest. 1985 Mar;75(3):1000-7 - PubMed
    1. Eur Respir J. 2006 Aug;28(2):339-45 - PubMed
    1. Thorax. 2010 Dec;65(12):1039-44 - PubMed
    1. Scand J Immunol. 2010 May;71(5):386-91 - PubMed
    1. Infect Immun. 2001 May;69(5):3435-7 - PubMed

Publication types

Substances

LinkOut - more resources

Feedback