¹H, ¹³C and ¹⁵N resonance assignment of the soluble form of the lipid-modified Azurin from Neisseria gonorrhoeae

Biomol NMR Assign. 2013 Oct;7(2):311-4. doi: 10.1007/s12104-012-9434-6. Epub 2012 Oct 16.

Abstract

Lipid-modified azurin (Laz) from Neisseria gonorrhoeae is a type 1 copper protein proposed to be the electron donor to several enzymes involved in the resistance mechanism to reactive oxygen and nitrogen species. Here we report the backbone and side-chain resonance assignment of Laz in the reduced form, which has been complete at 97%. The predicted secondary structure indicates that this protein belongs to the azurin subfamily of type 1 copper proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Azurin / chemistry*
  • Carbon Isotopes
  • Lipids / chemistry*
  • Molecular Sequence Data
  • Neisseria gonorrhoeae / metabolism*
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Structure, Secondary
  • Protons*
  • Solubility

Substances

  • Carbon Isotopes
  • Lipids
  • Nitrogen Isotopes
  • Protons
  • Azurin