Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato

PLoS One. 2012;7(10):e46763. doi: 10.1371/journal.pone.0046763. Epub 2012 Oct 5.

Abstract

Bacterial wilt, caused by the soil-borne bacterium Ralstonia solanacearum, is a lethal disease of tomato, but the molecular mechanisms of the host resistance responses to R. solanacearum remain unclear. In this study, we report the first work describing the transcriptome of cultivar resistance and susceptible tomato cultivar after inoculation with R. solanacearum. To elucidate the characteristics of resistance early in the interaction, we analyzed microarrays for resistant cultivar LS-89 and susceptible cultivar Ponderosa 1 day after stem inoculation. No change in gene expression was detected for Ponderosa, but expression levels of over 140 genes, including pathogenesis-related, hormone signaling and lignin biosynthesis genes, increased in LS-89. Expression of β-1,3-glucanase genes increased substantially. In an immunohistochemical study, glucanase in LS-89 accumulated in the xylem and pith tissues surrounding xylem vessels filled with R. solanacearum. The expression of these genes also increased in four other resistant cultivars, but changed little in four susceptible cultivars in response to R. solanacearum, suggesting that similar reactions occur in other cultivars. These gene expression profiles will serve as fundamental information to elucidate the molecular mechanisms in the resistance response to R. solanacearum in tomato.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Disease Resistance / genetics*
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Glycoside Hydrolases / genetics
  • Glycoside Hydrolases / metabolism
  • Host-Pathogen Interactions
  • Lignin / metabolism
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / immunology
  • Lycopersicon esculentum / microbiology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Stems / metabolism
  • Plant Stems / microbiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Ralstonia solanacearum / immunology
  • Ralstonia solanacearum / physiology*
  • Real-Time Polymerase Chain Reaction
  • Transcriptome*

Substances

  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Lignin
  • Glycoside Hydrolases

Grant support

This work was supported by research and development projects for application in promoting new policy of Agriculture Forestry and Fisheries from the Ministry of Agriculture, Forestry and Fisheries, Japan (21009) (URL of funder’s website: http://www.s.affrc.go.jp/docs/e/index.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.