Transforming growth factor-beta regulates the splicing pattern of fibronectin messenger RNA precursor

FEBS Lett. 1990 Feb 12;261(1):175-8. doi: 10.1016/0014-5793(90)80664-5.


Fibronectin (FN) polymorphism is caused by alternative splicing patterns in at least three regions (ED-A, ED-B and IIICS) of the primary transcript of a single gene. Using monoclonal antibodies, we previously demonstrated that transforming growth factor-beta (TGF-beta) preferentially increases the accumulation of the FN isoforms containing the ED-A sequence in cultured normal human fibroblasts [Balza et al., (1988) FEBS Lett. 228, 42-44]. To determine the basis of this effect, we have examined through S1 nuclease analysis, the levels of ED-A- and ED-B-containing mRNAs in cultured normal human skin fibroblasts before and after TGF-beta treatment. These experiments have shown that TGF-beta increases the relative amount of m-RNA for ED-A- and ED-B-containing FN isoforms. These data demonstrate that a growth factor may regulate the splicing pattern of a pre-mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • DNA Probes
  • Exons
  • Fibroblasts / metabolism
  • Fibronectins / genetics*
  • Humans
  • Lymphotoxin-alpha / pharmacology*
  • Nucleic Acid Hybridization
  • RNA Precursors / genetics*
  • RNA Splicing*
  • RNA, Messenger / genetics*
  • Single-Strand Specific DNA and RNA Endonucleases


  • DNA Probes
  • Fibronectins
  • Lymphotoxin-alpha
  • RNA Precursors
  • RNA, Messenger
  • Single-Strand Specific DNA and RNA Endonucleases