Members of the alpha-amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

FEBS Lett. 1990 Feb 12;261(1):85-8. doi: 10.1016/0014-5793(90)80642-v.


We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the alpha-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / analysis*
  • Allergens / immunology
  • Antigens / analysis*
  • Antigens / immunology
  • Asthma / immunology*
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immune Sera / immunology
  • Immunoglobulin E / immunology
  • Molecular Weight
  • Triticum / immunology*
  • alpha-Amylases / antagonists & inhibitors*


  • Allergens
  • Antigens
  • Immune Sera
  • Immunoglobulin E
  • alpha-Amylases