Clear-cut observation of PNA invasion using nanomechanical DNA origami devices

Chem Commun (Camb). 2012 Nov 28;48(92):11361-3. doi: 10.1039/c2cc36358e.

Abstract

Invasive binding event of PNA into DNA duplex was clearly observed both by atomic force microscope (AFM) imaging and electrophoretic mobility shift assay (EMSA) with the aid of nanomechanical DNA origami devices as 'single-molecule' visual probes, showing their potential as universal platform for the analysis of PNA invasion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Electrophoretic Mobility Shift Assay
  • Microscopy, Atomic Force
  • Nanotechnology
  • Nucleic Acid Hybridization
  • Peptide Nucleic Acids / chemistry*

Substances

  • Peptide Nucleic Acids
  • DNA