Ultrastructural localization of extracellular matrix proteins in liver biopsies using ultracryomicrotomy and immuno-gold labelling

Histopathology. 1990 Jan;16(1):53-8. doi: 10.1111/j.1365-2559.1990.tb01060.x.


We describe a technique for the localization of extracellular matrix proteins in wedge and needle biopsy specimens of human liver. Using ultra-thin (50-70 nm) sections of puncture perfusion fixed tissue, extracellular matrix proteins were localized using a protein A-gold labelling procedure. We obtained good preservation of ultrastructural detail and specific labelling for collagen types I, III and IV and fibronectin. The method represents a sensitive means of identifying the extracellular distribution and the cellular origin of these proteins in normal and diseased human liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy
  • Collagen / metabolism*
  • Extracellular Matrix / analysis*
  • Extracellular Matrix / ultrastructure
  • Fibronectins / metabolism*
  • Humans
  • Immunohistochemistry / methods*
  • Liver Diseases / metabolism*
  • Liver Diseases / pathology
  • Microtomy*
  • Staphylococcal Protein A*


  • Fibronectins
  • Staphylococcal Protein A
  • Collagen