An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction

Bioorg Med Chem. 2013 Mar 15;21(6):1465-9. doi: 10.1016/j.bmc.2012.09.047. Epub 2012 Sep 29.


We report the biochemical characterization of a new carbonic anhydrase (CA, EC, named SazCA, identified by translated genome inspection in Sulfurihydrogenibium azorense, a thermophilic bacterium from terrestrial hot springs of the Azores. SazCA is an α-CA showing kinetic parameters that make it the fastest enzyme of the CA family described so far. The biochemical properties, thermostability and inhibition of SazCA were compared with those of the thermophilic and mesophilic counterparts, demonstrating the special features of this unique enzyme.

MeSH terms

  • Amino Acid Sequence
  • Bacteria / enzymology*
  • Carbon Dioxide / metabolism*
  • Carbonic Anhydrases / classification
  • Carbonic Anhydrases / metabolism*
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Temperature
  • Water / metabolism*


  • Water
  • Carbon Dioxide
  • Carbonic Anhydrases