Expression of the murine Nr4a1 gene is controlled by three distinct genomic loci

Gene. 2013 Jan 10;512(2):517-20. doi: 10.1016/j.gene.2012.10.002. Epub 2012 Oct 16.

Abstract

The transcription factor NR4A1 belongs to the class of orphan nuclear receptors without known ligand which might control its activity. Here we examined its transcriptional regulation in response to elevated cAMP levels in HL-1 cardiac myocytes and in the heart in vivo. We report, that murine Nr4a1 is expressed from distinct promoters located upto -11.5 kb from the first coding exon. Whereas in HL-1 cells only two of the three distinct transcripts were induced by cAMP, in the heart all transcripts were regulated in response to β-adrenergic stimulation.

MeSH terms

  • Animals
  • Cell Line
  • Cyclic AMP / genetics
  • Cyclic AMP / metabolism
  • Gene Expression Regulation / physiology*
  • Genetic Loci / physiology*
  • Mice
  • Muscle Proteins / biosynthesis*
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / metabolism*
  • Nuclear Receptor Subfamily 4, Group A, Member 1 / biosynthesis*
  • Nuclear Receptor Subfamily 4, Group A, Member 1 / genetics
  • Promoter Regions, Genetic / physiology*
  • Transcription, Genetic / physiology

Substances

  • Muscle Proteins
  • Nr4a1 protein, mouse
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Cyclic AMP