Epigallocatechin-3-gallate inhibits angiotensin II and interleukin-6-induced C-reactive protein production in macrophages

Pharmacol Rep. 2012;64(4):912-8. doi: 10.1016/s1734-1140(12)70886-1.


Background: Consumption of green tea has been associated with health benefits against multiple diseases including cardiovascular diseases. However, the action mechanisms of green tea and its major ingredient epigallocatechin-3-gallate (EGCG) against cardiovascular diseases are still unclear. Emerging evidence has suggested a common role for C-reactive protein (CRP) in the pathogenesis of inflammation and atherosclerosis. Therefore, the effect of EGCG on angiotensin II (Ang II)- and interleukin-6 (IL-6)-induced CRP production in U937 macrophages and the possible mechanisms were observed.

Methods: U937 macrophages were cultured, and Ang II and IL-6 were used as stimulants for generation of CRP. U937 macrophages were preincubated with EGCG at 1, 3, 10 μM for 1 h prior to the stimulation. mRNA expression and protein level were determined by RT-PCR and ELISA, respectively. ROS production was observed by a fluorescence microscope.

Results: Pretreatment of macrophages with EGCG prior to the stimulation concentration-dependently inhibited Ang II- and IL-6-induced expression of CRP both in protein and mRNA levels. Meanwhile, EGCG reduced Ang II- and IL-6-stimulated generation of ROS in macrophages.

Conclusion: EGCG is able to inhibit Ang II- and IL-6-stimulated CRP expression in macrophages to produce an anti-inflammation by interfering with ROS generation. The finding is helpful to update understanding of anti-atherosclerotic effects of EGCG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / genetics
  • Angiotensin II / metabolism*
  • Anti-Inflammatory Agents / pharmacology
  • C-Reactive Protein / biosynthesis*
  • C-Reactive Protein / genetics
  • Cardiovascular Diseases / drug therapy
  • Cardiovascular Diseases / genetics
  • Cardiovascular Diseases / metabolism
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Cell Line, Tumor
  • Humans
  • Inflammation / drug therapy
  • Inflammation / genetics
  • Inflammation / metabolism
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • RNA, Messenger / genetics
  • Reactive Oxygen Species / metabolism
  • Tea
  • U937 Cells


  • Anti-Inflammatory Agents
  • Interleukin-6
  • RNA, Messenger
  • Reactive Oxygen Species
  • Tea
  • Angiotensin II
  • Catechin
  • C-Reactive Protein
  • epigallocatechin gallate