The manufacture and assessment of tissue microarrays: suggestions and criteria for analysis, with breast cancer as an example

J Clin Pathol. 2013 Mar;66(3):169-77. doi: 10.1136/jclinpath-2012-201091. Epub 2012 Oct 19.

Abstract

Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and high-throughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TMAs. Here we review some of the literature, using breast cancer as an example, to highlight good practice and pitfalls in the design and manufacture of TMAs. Issues, such as the size, number, spacing and layout of cores, as well as the assessment and reporting of studies using TMAs are addressed. We make some suggestions regarding these challenges, and propose a checklist of features that should be considered in order to stimulate debate and improve the quality of data produced by TMA analysis.

Publication types

  • Review

MeSH terms

  • Adenocarcinoma / diagnosis*
  • Adenocarcinoma / metabolism
  • Biomarkers, Tumor / metabolism
  • Breast Neoplasms / diagnosis*
  • Breast Neoplasms / metabolism
  • Female
  • Humans
  • Image Processing, Computer-Assisted
  • Sample Size
  • Specimen Handling / methods*
  • Tissue Array Analysis / methods*

Substances

  • Biomarkers, Tumor