Protein interactions of the transcription factor Hoxa1

BMC Dev Biol. 2012 Oct 22;12:29. doi: 10.1186/1471-213X-12-29.

Abstract

Background: Hox proteins are transcription factors involved in crucial processes during animal development. Their mode of action remains scantily documented. While other families of transcription factors, like Smad or Stat, are known cell signaling transducers, such a function has never been squarely addressed for Hox proteins.

Results: To investigate the mode of action of mammalian Hoxa1, we characterized its interactome by a systematic yeast two-hybrid screening against ~12,200 ORF-derived polypeptides. Fifty nine interactors were identified of which 45 could be confirmed by affinity co-purification in animal cell lines. Many Hoxa1 interactors are proteins involved in cell-signaling transduction, cell adhesion and vesicular trafficking. Forty-one interactions were detectable in live cells by Bimolecular Fluorescence Complementation which revealed distinctive intracellular patterns for these interactions consistent with the selective recruitment of Hoxa1 by subgroups of partner proteins at vesicular, cytoplasmic or nuclear compartments.

Conclusions: The characterization of the Hoxa1 interactome presented here suggests unexplored roles for Hox proteins in cell-to-cell communication and cell physiology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Green Fluorescent Proteins / metabolism
  • HEK293 Cells
  • Homeodomain Proteins / metabolism*
  • Humans
  • Mice
  • Pre-B-Cell Leukemia Transcription Factor 1
  • Protein Binding
  • Protein Interaction Maps*
  • Recombinant Fusion Proteins / metabolism
  • TNF Receptor-Associated Factor 1 / metabolism
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Homeodomain Proteins
  • Pbx1 protein, mouse
  • Pre-B-Cell Leukemia Transcription Factor 1
  • Recombinant Fusion Proteins
  • TNF Receptor-Associated Factor 1
  • Transcription Factors
  • homeobox A1 protein
  • Green Fluorescent Proteins