Inducing local DNA damage by visible light to study chromatin repair

DNA Repair (Amst). 2012 Dec 1;11(12):996-1002. doi: 10.1016/j.dnarep.2012.09.008. Epub 2012 Oct 22.

Abstract

Dynamics of DNA repair and recruitment of repair factors to damaged DNA can be studied by live cell microscopy. DNA damage is usually inflicted by a laser beam illuminating a DNA-interacting photosensitizer in a small area of the nucleus. We demonstrate that a focused beam of visible low intensity light alone can inflict local DNA damage and permit studies of DNA repair, thus avoiding potential artifacts caused by exogenous photosensitizers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Cell Nucleus / radiation effects
  • Chromatin / genetics
  • Chromatin / metabolism
  • Chromatin / radiation effects*
  • Color
  • DNA Damage*
  • DNA Repair
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Ethidium / adverse effects
  • Fluorescent Antibody Technique
  • Genome, Human / radiation effects
  • HeLa Cells
  • Histones / genetics
  • Histones / metabolism
  • Humans
  • Lasers / adverse effects
  • Light*
  • Oxidative Stress
  • Phosphorylation
  • Photosensitizing Agents / adverse effects
  • Time Factors
  • Transcription Factor TFIIH / genetics
  • Transcription Factor TFIIH / metabolism
  • X-ray Repair Cross Complementing Protein 1

Substances

  • Chromatin
  • DNA-Binding Proteins
  • H2AX protein, human
  • Histones
  • Photosensitizing Agents
  • X-ray Repair Cross Complementing Protein 1
  • Transcription Factor TFIIH
  • Ethidium