Measuring Protein Synthesis With SUnSET: A Valid Alternative to Traditional Techniques?

Exerc Sport Sci Rev. 2013 Apr;41(2):107-15. doi: 10.1097/JES.0b013e3182798a95.


Protein synthesis rates commonly are measured using isotopic tracers to quantify the incorporation of a labeled amino acid into muscle proteins. Here we provide evidence supporting our hypothesis that the nonisotopic SUnSET technique is a valid and accurate method for the measurement of in vivo changes in protein synthesis at the whole-muscle and single-muscle fiber levels.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Humans
  • Muscle Proteins / biosynthesis*
  • Muscle, Skeletal / metabolism*
  • Protein Synthesis Inhibitors*
  • Puromycin*


  • Muscle Proteins
  • Protein Synthesis Inhibitors
  • Puromycin