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, 39 (5), 452-6

The Effect of Phosphatidylcholine and Deoxycholate Compound Injections to the Localized Adipose Tissue: An Experimental Study With a Murine Model

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The Effect of Phosphatidylcholine and Deoxycholate Compound Injections to the Localized Adipose Tissue: An Experimental Study With a Murine Model

Yongjoon Noh et al. Arch Plast Surg.

Abstract

Background: Phosphatidylcholine (PPC) and deoxycholate (DCA) compound has been recently used for the purpose of partial lipolysis and is valued for its efficacy and lower invasiveness compared to liposuction and dermolipectomy used previously. In this article, the authors discuss the efficacy of the PPC dissolved in DCA via an experimental rat study model, along with suggesting a useful animal experimental model for the study of adipose tissue and lipolysis.

Methods: Bilateral inguinal fat pads of an experimental rat were elevated with the deep inferior epigastric vessel as the sole vascular pedicle. Normal saline was injected on one side as a control group and a PPC and DCA compound was injected on the other side. After 4 days, the rats were euthanized for microscopic tissue examination. The pathology was scored by a semiquantitative system in 4 categories: normal fat amount, fat necrosis, inflammatory activity, and stage of fibrosis. A Wilcoxon signed-rank test powered by SPSS packet program was used for statistical analysis and to determine significance.

Results: Microscopic examination was performed on the obtained samples, and the experimental data of all four categories showed significant histologic differences compared to the control group. All of the data also showed statistical significance by the Wilcoxon signedrank test (P<0.01).

Conclusions: In the inguinal fat pad rat model, the control group and the experimental group had a differed significantly in the amount of normal fat tissue, inflammation, necrosis, and fibrosis. We recommend the rat inguinal fat pad model used in this study, as it is likely to be useful in related research.

Keywords: Deoxycholate; Fat body; Lipolysis; Phosphatidylcholine.

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Inguinal fat pad of a rat Elevation of pedicled inguinal fat pad of a rat.
Fig. 2
Fig. 2
Gross photo of fat tissue Gross photo of inguinal fat pads after 4 days of experiments. PPC, phosphatidylcholine.
Fig. 3
Fig. 3
Result of the experiment Comparison of normal fat distribution, inflammation, necrosis, and fibrosis between saline-injected fat tissue and phosphatidylcholine (PPC)/deoxycholate injected fat tissue. All categories showed significant differences (P<0.01).
Fig. 4
Fig. 4
Microscopic view of saline injected fat tissue Microscopic examination of H&E stained fat tissue, ×100 magnification of saline injected fat tissue.
Fig. 5
Fig. 5
Microscopic view of PPC/DCA injected fat tissue Microscopic examination of H&E stained fat tissue, ×100 magnification of PPC-injected fat tissue. A histiocytic and giant cell reaction are seen with fat necrosis. Neutrophilic infiltrate is also seen, indicating an inflammatory response. PPC, phosphatidylcholine; DCA, deoxycholate.
Fig. 6
Fig. 6
Microscopic view of saline injected fat tissue Microscopic examination of Masson's trichrome-stained fat tissue, ×100 magnification of saline-injected fat tissue.
Fig. 7
Fig. 7
Microscopic view of PPC/DCA injected fat tissue Microscopic examination of Masson's trichrome-stained fat tissue, ×100 magnification of PPC-injected fat tissue. PPC, phosphatidylcholine; DCA, deoxycholate.

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