Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs

Methods Mol Biol. 2013;945:145-59. doi: 10.1007/978-1-62703-125-7_10.


The pulmonary alveolar epithelium, comprised of alveolar Type I (TI) and Type II (TII) cells, covers more than 99% of the internal surface area of the lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Epithelial Cells / cytology*
  • Flow Cytometry / methods*
  • Magnets
  • Pancreatic Elastase / metabolism
  • Perfusion
  • Pulmonary Alveoli / cytology*
  • Pulmonary Alveoli / metabolism
  • Rats
  • Rats, Sprague-Dawley


  • Pancreatic Elastase