Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs

Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. Epub 2012 Oct 23.

Abstract

Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target mimic (STTM) technology to block small RNA functions in plants and animals. STTM is a powerful technology complementing the previous target mimic (TM) in plants and the miRNA sponge, as well as the recently defined endogenous competing RNA (CeRNA) in animals. We expect STTM will not only be effective in blocking small RNA functions in plants but will also become a popular approach in animals.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Regulation, Plant
  • MicroRNAs* / antagonists & inhibitors
  • MicroRNAs* / chemistry
  • MicroRNAs* / genetics
  • Microsatellite Repeats / genetics
  • Plants* / genetics
  • RNA, Small Interfering / chemistry
  • Sequence Analysis, RNA

Substances

  • MIRN30b microRNA, human
  • MicroRNAs
  • RNA, Small Interfering