This large urban health unit identified a need for explicit, strategic, long-term organizational priority toward practical application of evidence in health promotion practice. Becoming a Best Practice Spotlight Organization (BPSO®) candidate provided an opportunity to systematically implement this commitment. The primary goals were to support incorporation of evidence-informed practice throughout the organization, increase interprofessional collaboration, and provide opportunities for knowledge exchange for staff. A mixed-methods evaluation consisting of three phases, including an analysis of previous evaluations, a survey of Champions, and an online focus group with the Steering Committee, demonstrated very positive outcomes. Staff reported increased incorporation of evidence in practice and program delivery. Collaboration and consultation amongst interdisciplinary staff across program areas also increased and staff responded very positively to increased opportunities for knowledge exchange. BPSO® candidacy opportunities should be used by health organizations to increase evidence-informed practice and inspire excellence in health promotion practice.