1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

BMB Rep. 2012 Oct;45(10):565-70. doi: 10.5483/bmbrep.2012.45.10.044.


We recently reported that a water extract of laurel or turmeric, 1,8-cineole enriched fractions, showed hypolipidemic activity in the zebrafish model. Therefore, the present study investigated the cineole's anti-oxidant and anti-inflammatory activities in lipoprotein metabolism in vitro and in vivo. Cineole had inhibitory effects on cupric ion-mediated oxidation of lipoproteins in general, while simultaneously enhancing ferric ion removal ability in high-density lipoprotein (HDL). Hypercholesterolemia was induced in zebrafish using cholesterol-feeding treatment, 4% cholesterol, for 3 weeks. After feeding with or without the addition of cineole, the results revealed that cineole possessed lipid-lowering and anti-inflammatory activities in hypercholesterolemic zebrafish. In addition, serum amyloid A and interleukin-6 levels were lowered and lipid accumulation was decreased in the liver. Conclusively, 1,8-cineole was found to have anti-oxidant activities in lipoprotein metabolism both in vitro and in vivo with simultaneous reduction of lipid accumulation in the liver of zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / pharmacology
  • Cholesterol, Dietary
  • Copper / chemistry
  • Cyclohexanols / pharmacology*
  • Eucalyptol
  • Glycosylation
  • Humans
  • Hypolipidemic Agents / pharmacology*
  • Interleukin-6 / metabolism
  • Lipid Metabolism / drug effects
  • Lipoproteins / chemistry
  • Lipoproteins / metabolism*
  • Lipoproteins, HDL / metabolism
  • Lipoproteins, LDL / metabolism
  • Monoterpenes / pharmacology*
  • Oxidation-Reduction
  • Zebrafish / metabolism


  • Anti-Inflammatory Agents
  • Antioxidants
  • Cholesterol, Dietary
  • Cyclohexanols
  • Hypolipidemic Agents
  • Interleukin-6
  • Lipoproteins
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Monoterpenes
  • Copper
  • Eucalyptol