Meiotic prophase requires proteolysis of M phase regulators mediated by the meiosis-specific APC/CAma1

Cell. 2012 Oct 26;151(3):603-18. doi: 10.1016/j.cell.2012.08.044.

Abstract

Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Cdc20 Proteins
  • Cell Cycle Proteins / metabolism*
  • Chromosome Segregation
  • Chromosomes, Fungal / metabolism
  • DNA-Binding Proteins / metabolism
  • Meiosis*
  • Metaphase
  • Prophase*
  • Protein-Serine-Threonine Kinases / metabolism
  • Proteolysis
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Spindle Apparatus
  • Transcription Factors / metabolism
  • Ubiquitin-Protein Ligase Complexes / metabolism*

Substances

  • Ama1 protein, S cerevisiae
  • Cdc20 Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • NDD1 protein, S cerevisiae
  • NDT80 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • Protein-Serine-Threonine Kinases
  • CDC5 protein, S cerevisiae