Abstract
CMY-2 Β-lactamase is an important cause of Β-lactam resistance in Enterobacteriaceae and constitutes the most widespread pAmpC. Although CMY-2 has been previously recognized in our region, the real prevalence and epidemiology of this resistance marker was uncertain. During August-October 2009, we conducted a multicenter, prospective study to determine pAmpC prevalence and to characterize CMY-2 producing Escherichia coli associated plasmids. Plasmid-encoded AmpC prevalence was 0.9 % in enterobacteria in this period, being CMY-2 prevalent and to a lesser extent DHA. Molecular typing of CMY-2- producing Escherichia coli isolates showed several lineages. Moreover, replicon typing of cmy-2- containing plasmids displayed a broad diversity in Inc/cmy-2 links. Therefore, association of cmy-2 with specific transposon elements may be responsible for the spread of this resistance marker in Enterobacteriaceae.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Anti-Bacterial Agents / therapeutic use
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Argentina / epidemiology
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Bacterial Proteins / analysis*
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DNA Primers
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Disk Diffusion Antimicrobial Tests
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Drug Resistance, Multiple, Bacterial / genetics*
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / genetics
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae Infections / drug therapy
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Enterobacteriaceae Infections / epidemiology*
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Enterobacteriaceae Infections / microbiology
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli Infections / drug therapy
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Escherichia coli Infections / epidemiology
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Escherichia coli Infections / microbiology
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Humans
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Phylogeny
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Prospective Studies
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R Factors / chemistry*
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Species Specificity
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beta-Lactam Resistance / genetics*
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beta-Lactamases / analysis*
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA Primers
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beta-lactamase CMY-2
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AmpC beta-lactamases
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beta-Lactamases