IRIS iQ200 workstation as a screen for performing urine culture

Diagn Microbiol Infect Dis. 2013 Jan;75(1):5-8. doi: 10.1016/j.diagmicrobio.2012.08.026. Epub 2012 Oct 24.


We cultured 1000 urines and tested them with the iQ200 Workstation (Iris Diagnostics, Chatsworth, CA, and Arkray, Edina, MN, USA) for leukocyte esterase and nitrite (LE/N), white blood cells (WBC), all small particles (ASP), bacteria, and yeast. Positive culture grew ≥ 10(4) CFU/mL of 1 or 2 uropathogens. Method A assessed LE/N, WBCs ≥ 6/μL, and bacteria/yeast. Method B assessed WBCs ≥ 6/μL, bacteria/yeast, and ASP ≥ 10,000. Each was compared to culture results, clinician's diagnosis (CD), or expert review (ER). Sensitivities by culture were 88.7% (Method A) and 88.3% (Method B), with negative predictive values (NPVs) of 94.4% and 94.2%, respectively. Sensitivity and NPV increased for comparison to the CD (99.6%) and NPVs of 99.0% and 99.3% compared to ER. ASP did not enhance specificity, sensitivity, or NPV. The iQ200 Workstation performed well by any standard, thus providing a reliable system by which to improve the use of laboratory resources.

MeSH terms

  • Bacteria / isolation & purification
  • Bacterial Load
  • Carboxylic Ester Hydrolases / analysis
  • Colony Count, Microbial
  • Female
  • Humans
  • Leukocyte Count
  • Male
  • Microbiological Techniques / methods*
  • Nitrites / analysis
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Urinary Tract Infections / diagnosis*
  • Urinary Tract Infections / microbiology*
  • Urine / microbiology*
  • Yeasts / isolation & purification


  • Nitrites
  • leukocyte esterase
  • Carboxylic Ester Hydrolases