P-body components LSM1, GW182, DDX3, DDX6 and XRN1 are recruited to WNV replication sites and positively regulate viral replication

Virology. 2013 Feb 5;436(1):1-7. doi: 10.1016/j.virol.2012.09.041. Epub 2012 Oct 24.


In mammalian cells, proteins involved in mRNA silencing and degradation localize to discrete cytoplasmic foci called processing or P-bodies. Here we show that microscopically visible P-bodies are greatly diminished following West Nile viral infection, but the component proteins are not depleted. On the other hand, many P-body components including LSM1, GW182, DDX3, DDX6 and XRN1, but not others like DCP1a and EDC4 are recruited to the viral replication sites, as evidenced by their colocalization at perinuclear region with viral NS3. Kinetic studies suggest that the component proteins are first released from P-bodies in response to WNV infection within 12 h post-infection, followed by recruitment to the viral replication sites by 24-36 h post-infection. Silencing of the recruited proteins individually with siRNA interfered with viral replication to varying extents suggesting that the recruited proteins are required for efficient viral replication. Thus, the P-body proteins might provide novel drug targets for inhibiting viral infection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Cell Line, Tumor
  • Cricetinae
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism
  • Exoribonucleases / genetics
  • Exoribonucleases / metabolism
  • Geographic Information Systems
  • HeLa Cells
  • Humans
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • RNA Helicases / metabolism
  • RNA Interference
  • RNA, Small Interfering
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Serine Endopeptidases / metabolism
  • Viral Nonstructural Proteins / metabolism
  • Virus Replication / physiology*
  • West Nile Fever / metabolism*
  • West Nile virus / growth & development*
  • West Nile virus / metabolism*


  • Autoantigens
  • LSM1 protein, human
  • Microtubule-Associated Proteins
  • NS3 protein, flavivirus
  • Proto-Oncogene Proteins
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • TNRC6A protein, human
  • Viral Nonstructural Proteins
  • Exoribonucleases
  • XRN1 protein, human
  • Serine Endopeptidases
  • DDX3X protein, human
  • DDX6 protein, human
  • DEAD-box RNA Helicases
  • RNA Helicases