Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for lysozyme purification from chicken egg white

Artif Cells Nanomed Biotechnol. 2013 Feb;41(1):13-20. doi: 10.3109/10731199.2012.696067. Epub 2012 Oct 30.

Abstract

The purpose of this article is to synthesize poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPHEMATrp] magnetic nanoparticles for lysozyme purification from chicken egg white. mPHEMATrp nanoparticles (38 nm in diameter) were synthesized by surfactant-free emulsion polymerization. Specific surface area of the mPHEMATrp nanoparticles was calculated to be 896 m2/g. Elemental analysis of mPHEMATrp nanoparticles was estimated in the range of 5.7-48.3 µmol MATrp/g. The maximum lysozyme adsorption capacity of the mPHEMATrp nanoparticles was 376.1 mg/g. The mPHEMATrp nanoparticles could be used five times without decreasing the lysozyme adsorption capacity significantly. The results indicate that the mPHEMATrp nanoparticles promise high selectivity for lysozyme purification.

MeSH terms

  • Adsorption
  • Animals
  • Chickens
  • Egg White / chemistry*
  • Magnetite Nanoparticles / chemistry*
  • Muramidase / isolation & purification*
  • Polyhydroxyethyl Methacrylate / analogs & derivatives*
  • Polyhydroxyethyl Methacrylate / chemical synthesis
  • Polyhydroxyethyl Methacrylate / metabolism
  • Sensitivity and Specificity
  • Tyrosine / analogs & derivatives*
  • Tyrosine / chemical synthesis
  • Tyrosine / metabolism

Substances

  • Magnetite Nanoparticles
  • poly(hydroxyeethyl methacrylate-N-methacryloyl-tyrosine methyl ester)
  • Polyhydroxyethyl Methacrylate
  • Tyrosine
  • hen egg lysozyme
  • Muramidase