A Conserved Cleavage-Site Motif in Chloroplast Transit Peptides

FEBS Lett. 1990 Feb 26;261(2):455-8. doi: 10.1016/0014-5793(90)80614-o.

Abstract

A collection of 32 stroma-targeting chloroplast transit peptides with known cleavage sites have been analysed in terms of amino acid preferences in the vicinity of the processing site. A loosely conserved consensus motif (Val/Ile)-X-(Ala/Cys) decreases Ala is found in the majority of the transit peptides. About 30% of the sequences have a perfect match to the consensus. When such a match is found, there is a 90% probability that it specifies the correct cleavage site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analysis
  • Amino Acid Sequence
  • Arginine / analysis
  • Carrier Proteins / analysis
  • Carrier Proteins / metabolism*
  • Chloroplasts / analysis*
  • Cysteine / analysis
  • Isoleucine / analysis
  • Molecular Sequence Data
  • Plant Proteins / analysis
  • Plant Proteins / metabolism*
  • Valine / analysis

Substances

  • Carrier Proteins
  • Plant Proteins
  • Isoleucine
  • Arginine
  • Valine
  • Cysteine
  • Alanine