Estimating volume in biological structures

Cold Spring Harb Protoc. 2012 Nov 1;2012(11):1129-39. doi: 10.1101/pdb.top071787.


Estimates of volume can be useful in comparative and experimental studies of tissues and organs. In this article, a simple procedure for making unbiased estimates of total volume using Cavalieri's principle is described. This is followed by a description of how point probes can be used to make unbiased estimates of the areas of sectional profiles and how, when combined with Cavalieri's principle, point counting can be used to make an unbiased estimate of the volume of a structural feature.

MeSH terms

  • Biometry / methods*
  • Image Processing, Computer-Assisted / methods*
  • Models, Theoretical*