Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria

Appl Environ Microbiol. 2013 Jan;79(2):718-21. doi: 10.1128/AEM.02926-12. Epub 2012 Nov 2.


We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Transposable Elements
  • Gene Expression*
  • Genetic Vectors
  • Genetics, Microbial / methods*
  • Gram-Negative Bacteria / genetics*
  • Host Specificity
  • Mutagenesis, Insertional / methods*
  • Promoter Regions, Genetic


  • DNA Transposable Elements